Coding
DNA Ligase

Part:BBa_K2468000:Design

Designed by: Ayesha Ellahi   Group: iGEM17_UIUC_Illinois   (2017-10-25)


Thermotoga maritima DNA Ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 404
    Illegal BglII site found at 1544
    Illegal BglII site found at 1800
    Illegal BamHI site found at 713
    Illegal BamHI site found at 1221
    Illegal XhoI site found at 1513
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1529
    Illegal BsaI.rc site found at 331


Design Notes

No specific design considerations.


Source

The source of this part is from the genomic sequence of Thermotoga maritime. Blasting the sequence will result in a high identity match with regions in the genome of T. maritima.

References

1. https://www.ncbi.nlm.nih.gov/nuccore/CP011108.1?from=1306720&to=1307097&sat=4&sat_key=139234589